Enzyme Immunoassays

Enzyme immunoassay is based on the application of enzyme for detection and quantification of specifically labeled immunocomplex. Greater specificity, cheapness, and independency on radioactive tracers have made enzyme immunoassay popular over alternative techniques for analyzing a variety of samples. Apart from purity, stability, and turnover number, however, the practicability of conjugating the enzyme and its effect on enzyme activity restricts the choice of enzymes. Current developments in this field include mechanization of execution; fabrication of sensitive optical detectors, amplification devices, and new solid matrices; automated computer-based multiplex systems; and integration of knowledge from other disciplines of science.