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Mitigation of radiation-induced oxidative stress by methanolic extract of Tragia involucrata in swiss albino mice
- Source :
- Pharmacognosy Research. 11:236
- Publication Year :
- 2019
- Publisher :
- EManuscript Technologies, 2019.
-
Abstract
- Background: Tragia involucrata L. has been used in Indian traditional medicine since centuries to treat various ailments. The plant remains unexplored for its radioprotective properties and hence the present study. Objective: The main objective of the study is to ascertain the radioprotective effects of T. involucrata methanolic extract (TME) in Swiss albino mice against whole-body gamma radiation. Materials and Methods: The acute toxicity of TME was evaluated. The optimal protective dose of TME against radiation-induced mortality in mice was determined by survival analysis using the Kaplan–Meier curves. To understand the mechanism of radioprotection, TME was tested for hematological changes and antioxidant levels in mice by injecting100 mg/kg body weight intraperitoneally for 5 days and irradiated with a sublethal dose of 6 Gy gamma radiation. Results: The lethal dose 50%/of TME was about 645.65 mg/kgbw. The pretreatment of mice with 100 mg/kgbw of TME increased its survivability to 30% compared with the radiation control group and hence is considered as a radioprotective dose. The lethal dose 50%/30% of the irradiation alone group was found to be 9 Gy and 10.35 Gy for the TME + irradiation group. Hence, the dose reduction factor was about 1.15. The radiation decreased the hematological parameters in the blood, but on pretreatment with TME (100 mg/kgbw), increase in levels was noted. The antioxidant enzyme levels in the mice liver homogenate were found to be replenished in the test group compared with the radiation control. Conclusion: The present study indicates the protective role of TME against gamma radiation-induced mortality and oxidative stress. Hence, it is proposed as a candidate for radioprotection.
- Subjects :
- Pharmacology
Antioxidant
biology
Test group
business.industry
medicine.medical_treatment
0211 other engineering and technologies
Radiation induced
02 engineering and technology
medicine.disease_cause
biology.organism_classification
01 natural sciences
Median lethal dose
Acute toxicity
0104 chemical sciences
Ionizing radiation
010404 medicinal & biomolecular chemistry
Tragia involucrata
021105 building & construction
Drug Discovery
medicine
sense organs
business
Oxidative stress
Subjects
Details
- ISSN :
- 09748490
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Pharmacognosy Research
- Accession number :
- edsair.doi...........8b624b96609c3df34cd4fcf33ce7d9e2