Application of the theory of kubelka and munk to densitometry

The theory of Kubelka and Munk is evaluated for simultaneous measurement of reflectance and transmittance as a densitometric method. A relation yielding the amount of substance in a t.l.c. spot, as a function of its reflectance and transmittance, is derived. A densitometer operating according to this relation is described. To check the validity of the derived relation, measurements were made on spots of Sudan yellow developed with chloroform on silica layers. The results show a satisfactory proportionality between apparatus response and amount of substance applied.