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DNA content analysis of smooth pigweed (Amaranthus hybridus) and tall waterhemp (A. tuberculatus): implications for hybrid detection

Authors :
Mark R. Jeschke
A. Lane Rayburn
Patrick J. Tranel
Source :
Weed Science. 51:1-3
Publication Year :
2003
Publisher :
Cambridge University Press (CUP), 2003.

Abstract

Prior research indicated that analysis of nuclear DNA content by flow cytometry could be used to distinguish smooth pigweed × tall waterhemp hybrids when the parent lines are known. Flow cytometry was performed on nuclei isolated from several Illinois populations of smooth pigweed and tall waterhemp. The smooth pigweed and tall waterhemp analyzed had nonoverlapping 2C nuclear DNA content values, with mean values of 1.04 and 1.34 pg, respectively. The consistent difference in DNA content observed between the two species indicates that DNA content analysis can be used to distinguish their hybrid progeny in natural populations. Nomenclature: Smooth pigweed, Amaranthus hybridus L. AMACH; tall waterhemp, Amaranthus tuberculatus (Moq.) Sauer AMATU.

Details

ISSN :
15502759 and 00431745
Volume :
51
Database :
OpenAIRE
Journal :
Weed Science
Accession number :
edsair.doi...........8a78d7783c15d9ab7551142d64987b19
Full Text :
https://doi.org/10.1614/0043-1745(2003)051[0001:dcaosp]2.0.co;2