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MICROFLUOROMETRIC MEASUREMENT OF DNA IN EUDORINA ELEGANS AND E. CALIFORNICA (CHOROPHYCEAE)1

Authors :
Ron Anderson
C. Lindley Kemp
Gordon Doyle
Source :
Journal of Phycology. 15:464-465
Publication Year :
1979
Publisher :
Wiley, 1979.

Abstract

The fluorescent dye 2,5-bis (4′-aminophenyl (1′))-1,3,4-oxidiwle (BAO) was compared to the Feulgen procedure as a method for quantitative comparison of DNA levels in two species of Eudorina. The BAO procedure proved to be specific and as quantitative as the Feulgen procedure, and was more convenient and rapid. The DNA level of the nuclei of gonidial cells of Eudorina elegans Ehrenberg and E. californica (Shaw) Goldstein doubled prior to each division. The DNA level of the somatic, usually non-dividing nuclei of E. californica did not vary from the IC (haploid) level.

Details

ISSN :
00223646
Volume :
15
Database :
OpenAIRE
Journal :
Journal of Phycology
Accession number :
edsair.doi...........8a5955cf1c47157c881ccb467186fdd7