Complement fixation to a 50% hemolysis end point with protein antigen adsorbed on paper, and its action on anti-cancer sera

Protein antigens fixed on paper in the form of azocompounds or by simple drying at room temperature have a high serological activity and specifically fix the complement in the presence of a corresponding anti-serum. This was established by the method of quantitative reaction of complement fixation (50% titer). Complement fixation test (50% titer) for “paper” antigens gives an opportunity of using different antigens in the same test and replacing them during the experiment. This permits the use of antigens adsorbed on paper and the quantitative complement fixation test for investigation of the optimal conditions of obtaining the specific antisera.