THE ENHANCEMENT OF COLLAGEN SYNTHESIS PROCESS ON DIABETIC WOUND BY MERREMIA MAMMOSA (LOUR.) EXTRACT FRACTION

Objective: This research aimed to evaluate the effect of fractionation of Merremia mammosa Lour. (Mm (Lour.)) extract on diabetic wound healing by observing collagen synthesis process and to search the most potent fraction.Methods: Wistar rats were divided into five groups (n=5), i.e., K-(negative control), K+ (positive control), K1 (ethyl acetate fraction), K2 (water fraction), and K3 (n-hexane fraction). The Mm (Lour.) was extracted with ethanol 70%, then fractionated by using three solvents which have different polarity. The rats were adapted in 7 days, then induced into diabetic by streptozotocin dose 40mg/kg body weight. The wound was made by Morton excision method. Treatment was given every two days and skin biopsy was done on day 11. Analysis of collagen density was done by photomicrograph of histopathology preparations in Masson’s trichome stained by using trinocular microscope with 400x magnification in 6 fields of view, then processed by imageJ software and analyzed by appropriate statistic tool.Result: The results of this research showed that fractionation of Mm (Lour.) extract significantly enhanced diabetic wound healing based on macroscopic (percentage of wound healing) and collagen density with p-value