Effectiveness of Garlic Water Extract Gel (Allium sativum. L) Against Necrotic Factor Alfa (TNF-α) Tumors and Mouth Ulcer Diameter in Rats

Oral ulcers are pathological conditions that are characterized by loss of epithelial tissue, causing inflammation. During inflammation, the inflammatory mediator is TNF will increase. Therefore, drugs are needed to suppress TNF-levels to suppress inflammation. One of the herbal ingredients that can suppress inflammation is garlic. In vivo laboratory experimental research with pre-post systems. The samples used were 36 male Wistar white rats divided into 6 groups (6 individuals / groups), namely the group without drug treatment (as pre-treatment data), negative control group namely HPMC gel base, positive control given benzydamin, group treatment I was given a garlic water extract gel 20%, treatment group II was given a garlic water extract gel 40%, treatment group III were given a garlic water extract gel 80%. The drug is given topically 2 times a day for 4 days. Making oral ulcers is done by placing filter paper that has been soaked in a 50% acetic acid solution in the labial area of the mandibular gingival tissue. Measurement of oral ulcers was performed on the 1st day after the manufacture of oral ulcers and the 5th day. On the 5th day all the mice were sacrificed to be taken gingival tissue and TNF-levels were measured. using ELISA. Data analysis using SPSS version 24 and One Way Anova test with 5% specification followed by Post Hoc Test using LSD test and Games Howell. Analysis using One Way Anova showed that there were significant differences in TNF-levels and the diameter of the oral ulcer (p