Effect of DNA in controlling the H-dimeric sites of Nile Blue Chloride (NBC) in ultra-thin film

The focus of the present work is to investigate the interaction of NBC with DNA and also to study the control of aggregation of NBC in solution as well as in LBL film by incorporating DNA. Nile Blue, a benzophenoxazine class of dye, is a well-known DNA binding probe. DNA can organize various organic molecules because it has a characteristic structure, such as the double strand with hydrogen bondings and molecules can intercalate into the stacking base pairs. On the other hand benzophenoxazine class of dyes exhibit relatively low systemic toxicity and some of them retard tumour growth. Compared to other conventional intercalators, the use of NBC has a merit of having low toxicity and comparable sensitivity for DNA quantification. UV-Vis absorption spectroscopic and fluorescence spectroscopic techniques have been employed to study the formation and control of aggregation in ultrathin films.