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Evaluation of the possible therapeutic role of omega-3 on ankle joint and lung in a model of rheumatoid arthritis in rats: A histological and immunohistochemical study
- Source :
- Egyptian Journal of Histology. 41:1-14
- Publication Year :
- 2018
- Publisher :
- Egypts Presidential Specialized Council for Education and Scientific Research, 2018.
-
Abstract
- Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory systemic autoimmune disease. Lung disease has beenrecognized as a common complication of RA. Omega-3 is an essential fatty acid with wide range of activity.Aim of the work: To investigate the possible therapeutic role of omega-3 free fatty acid on the structure of ankle joint andlung in a model of adjuvant-induced RA in rats.Materials and Methods: Thirty adult male albino rats were divided into three groups; Group I served as a control group.Group II was used as a model of RA. Rats of this group were given single subcutaneous injection of 0.1ml of completeFreund adjuvant in their hind paws. Group III (omega 3-treated group), omega-3 was given in as a dose of 300 mg/kg orallyadministered after two weeks of RA induction and continued daily for another two weeks. The ankle joint and the lungs fromall groups were dissected out after two and four weeks and processed for light microscopic examination. Histomorphometricstudies and statistical analysis were also done.Results: RA was confirmed histologically in the ankle joint after two weeks and was aggravated after four weeks. Erosionof articular cartilage and mononuclear cellular infiltration in the synovium were noticed in both time points. Moreover, thelungs after two weeks showed focal inflammatory cellular infiltration and pulmonary fibrosis that were more evident afterfour weeks of untreated RA. This was associated with significant increase in the mean area percentage of collagen fibers andnumber of cells positive for TNF-α immunohistochemical reaction compared to the control group. Omega-3 treated groupillustrated improvement of both ankle and lung structures.Conclusion: Omega-3 significantly ameliorated the structural damage of ankle joint and lung after RA induction.
- Subjects :
- 030203 arthritis & rheumatology
0301 basic medicine
Pathology
medicine.medical_specialty
Lung
business.industry
medicine.disease
Cellular infiltration
03 medical and health sciences
Subcutaneous injection
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Rheumatoid arthritis
Pulmonary fibrosis
Medicine
Tumor necrosis factor alpha
Ankle
business
Complication
Subjects
Details
- ISSN :
- 20902417
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Egyptian Journal of Histology
- Accession number :
- edsair.doi...........87150beff8454dbe2b369d758e273c9f