High performance liquid chromatographic behaviour of several barbiturates on a newly developed high purity silica column

A simple isocratic reversed phase HPLC method for the separation of a series of barbiturates is described. The column used is a newly developed synthetic silica known as Symmetry Clg (Waters Corp., Milford, MA, USA) and the mobile phase consists of 50 mM KH2PO4 (pH 4.55): acetonitrile water (20:30:50:). The barbiturates studied are monitored by UV detection at 225 nm. Detailed methodology and the advantage of the Symmetry C18 reversed phase is presented.