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Two-point optical manipulation reveals mechanosensitive remodeling of cell–cell contacts in vivo

Authors :
Kenji Nishizawa
Shao-Zhen Lin
Claire Chardès
Jean-François Rupprecht
Pierre-François Lenne
Source :
Proceedings of the National Academy of Sciences. 120
Publication Year :
2023
Publisher :
Proceedings of the National Academy of Sciences, 2023.

Abstract

Biological tissues acquire reproducible shapes during development through dynamic cell behaviors. Most of these behaviors involve the remodeling of cell–cell contacts. During epithelial morphogenesis, contractile actomyosin networks remodel cell–cell contacts by shrinking and extending junctions between lateral cell surfaces. However, actomyosin networks not only generate mechanical stresses but also respond to them, confounding our understanding of how mechanical stresses remodel cell–cell contacts. Here, we develop a two-point optical manipulation method to impose different stress patterns on cell–cell contacts in the early epithelium of the Drosophila embryo. The technique allows us to produce junction extension and shrinkage through different push and pull manipulations at the edges of junctions. We use these observations to expand classical vertex-based models of tissue mechanics, incorporating negative and positive mechanosensitive feedback depending on the type of remodeling. In particular, we show that Myosin-II activity responds to junction strain rate and facilitates full junction shrinkage. Altogether our work provides insight into how stress produces efficient deformation of cell–cell contacts in vivo and identifies unanticipated mechanosensitive features of their remodeling.

Subjects

Subjects :
Multidisciplinary

Details

ISSN :
10916490 and 00278424
Volume :
120
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences
Accession number :
edsair.doi...........85eac1645901800503452a9009a32795
Full Text :
https://doi.org/10.1073/pnas.2212389120