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Brassinosteroids mediate the effect of soil-drying during meiosis on spikelet degeneration in rice
- Source :
- Environmental and Experimental Botany. 169:103887
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- This study investigated whether and how brassinosteroids (BRs) mediate the effect of soil-drying during meiosis on spikelet degeneration in rice (Oryza sativa L.). A field-grown rice cultivar was exposed to three soil moisture treatments during meiosis: well-watered (WW), moderate soil-drying (MD) and severe soil-drying (SD). Compared with the WW treatment, the MD treatment enhanced BRs biosynthesis, reduced spikelet degeneration, and increased grain yield. The SD treatment had the opposite effects. Changes in the levels of adenosine triphosphate (ATP), energy charge, activities of enzymes involved in energy metabolism, including cytochrome C oxidase (CCO) and succinate dehydrogenase (SDH), and total antioxidant capacity (T-AOC) were consistent with those in BRs levels, whereas the hydrogen peroxide (H2O2) content, vacuolar processing enzyme genes (OsVPE2 and OsVPE3) expression levels and the degree of nuclear DNA fragmentation showed the opposite trend. Knockdown of the BRs synthesis gene OsD11 or application of a BRs biosynthesis inhibitor to young panicles vastly decreased the BRs and ATP contents, energy charge, CCO and SDH activities, and T-AOC in young panicles but increased the H2O2 content, OsVPE2 and OsVPE3 expression, and spikelet degeneration compared to the control, and the opposite effects were observed when exogenous BRs were applied. Therefore, BRs likely mediate the effect of soil-drying on spikelet degeneration, and elevated BRs levels in rice panicles inhibit spikelet degeneration under moderate soil-drying during meiosis by elevating antioxidant capacity and energy status.
- Subjects :
- 0106 biological sciences
0301 basic medicine
medicine.medical_specialty
Plant Science
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Internal medicine
medicine
Cytochrome c oxidase
Energy charge
Fragmentation (cell biology)
Ecology, Evolution, Behavior and Systematics
chemistry.chemical_classification
Oryza sativa
biology
Chemistry
Succinate dehydrogenase
fungi
food and beverages
030104 developmental biology
Enzyme
Endocrinology
biology.protein
Agronomy and Crop Science
Adenosine triphosphate
010606 plant biology & botany
Subjects
Details
- ISSN :
- 00988472
- Volume :
- 169
- Database :
- OpenAIRE
- Journal :
- Environmental and Experimental Botany
- Accession number :
- edsair.doi...........8598e298f602404d1c379f7fe1735670