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Late-stage deuteration of13C-enriched substrates forT1prolongation in hyperpolarized13C MRI
- Source :
- Chemical Communications. 54:5233-5236
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry (RSC), 2018.
-
Abstract
- A robust and selective late-stage deuteration methodology was applied to 13C-enriched amino and alpha hydroxy acids to increase spin–lattice relaxation constant T1 for hyperpolarized 13C magnetic resonance imaging. For the five substrates with 13C-labeling on the C1-position ([1-13C]alanine, [1-13C]serine, [1-13C]lactate, [1-13C]glycine, and [1-13C]valine), significant increase of their T1 was observed at 3 T with deuterium labeling (+26%, 22%, +16%, +25% and +29%, respectively). Remarkably, in the case of [2-13C]alanine, [2-13C]serine and [2-13C]lactate, deuterium labeling led to a greater than four fold increase in T1. [1-13C,2-2H]alanine, produced using this method, was applied to in vitro enzyme assays with alanine aminotransferase, demonstrating a kinetic isotope effect.
- Subjects :
- Alanine
biology
Stereochemistry
Chemistry
Metals and Alloys
Hyperpolarized 13c
General Chemistry
010402 general chemistry
01 natural sciences
Catalysis
In vitro
Enzyme assay
030218 nuclear medicine & medical imaging
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Serine
03 medical and health sciences
0302 clinical medicine
Deuterium
Valine
Kinetic isotope effect
Materials Chemistry
Ceramics and Composites
biology.protein
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi...........84dd8d47df796cbeda17b6f2979f1ea4