Restoration of Ribozyme Activity by Switching the Positions of Cy3 and Cy5 in the Ribozymes Inactivated with the Fluorescent Dyes

Ribozyme119 derivatives from the Kin.46 self-thiopho-sphorylating ribozyme by internal deletions has 3 strands; a left high (LH) strand, a lower (lw) strand and an activating oligomer (AO) with 18 nucleotide (A), and has 4 strands; a 7-mer, an upper (up) strand, a lower (lw) strand and an activating oligomer (AO) with 18 nucleotide (B). For a lw strand, dyes (Cy3 and Cy5) were incorporatedto the 5’-end during transcription with the class II promoter using AMP-dye-AMP as a primer. Dye-labeled AO was purchased.