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Coalescence of functional gold and monodisperse silver nanoparticles mediated by black Panax ginseng Meyer root extract
- Source :
- International Journal of Nanomedicine. 11:6621-6634
- Publication Year :
- 2016
- Publisher :
- Informa UK Limited, 2016.
-
Abstract
- A rapid biological synthesis of multifunctional gold nanoparticle (AuNp) and monodisperse silver nanoparticle (AgNp) was achieved by an aqueous extract of black Panax ginseng Meyer root. The physicochemical transformation into black ginseng (BG) greatly enhanced the pharmacological activities of white ginseng and its minor ginsenoside content. The optimal temperature conditions and kinetics of bioreduction were investigated. Formation of BG-AuNps and BG-AgNps was verified by ultraviolet-visible spectrophotometry at 548 and 412 nm, respectively. The biosynthesized BG-AgNps were spherical and monodisperse with narrow distribution, while BG-AuNps were icosahedral-shaped and moderately polydisperse. Synthesized nanoparticles exhibited long-term stability in buffers of pH 7.0-8.0 and biological media (5% bovine serum albumin) at an ambient temperature and at 37°C. BG-AgNps showed effective antibacterial activity against Escherichia coli and Staphylococcus aureus. BG-AuNps and BG-AgNps demonstrated increased scavenging activity against 2,2-diphenyl-1-picrylhydrazyl free radicals. In addition, BG-AuNps and BG-AgNps were nontoxic to HaCaT and MCF-7 cells; the latter showed no cytotoxicity at concentrations lower than 10 µg/mL. At higher concentrations, BG-AgNps exhibited apparent apoptotic activity in MCF-7 breast cancer cell line through reactive oxygen species generation and nuclear fragmentation.
- Subjects :
- 0301 basic medicine
Materials science
Biophysics
Pharmaceutical Science
Nanoparticle
Bioengineering
02 engineering and technology
Silver nanoparticle
Biomaterials
03 medical and health sciences
Ginseng
chemistry.chemical_compound
Drug Discovery
Bovine serum albumin
biology
Traditional medicine
Organic Chemistry
General Medicine
021001 nanoscience & nanotechnology
HaCaT
030104 developmental biology
chemistry
Ginsenoside
Colloidal gold
biology.protein
0210 nano-technology
Antibacterial activity
Nuclear chemistry
Subjects
Details
- ISSN :
- 11782013
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi...........843a18208a616fca4f7c016592e30872