Proteomic approach for protein expression profiling of promyelocytic leukemia cell line in response to infection with intracellular parasitic bacteria of emerging infectious diseases using two dimensional difference gel electrophoresis

Human granulocytic anaplasomsis and human monocytic ehrlichiosis are tick-borne emerging infectious diseases which are caused by Anaplasma phagocytophilum and Ehrlichia chaffeensis. These bacteria are obligatory intracellular parasites with tropism for hematopoietic cells. Here, we introduced analysis of protein expression profiles of HL-60 host cells in the response to infection with those bacteria by proteomic approach using fluorescence-prelabeled two dimensional difference gel electrophoresis to clarify the molecular mechanisms of the intracellular parasitism. Three experiments were designed for this purpose: differential analysis of infection at late (i) or early (iii) stages, and time-course analysis postinfection (ii). The detailed technical approaches, advantage of this procedure, and technical improvements desired were described and discussed.