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Opposite modulation of functional recovery following contusive spinal cord injury in mice with oligodendrocyte-selective deletions of Atf4 and Chop/Ddit3

Authors :
Yonglin Gao
George Wei
Michael D. Forston
Emily R. Hodges
Darlene Burke
Kariena Andres
Johnny Morehouse
Christine Armstrong
Charles Glover
Lukasz P. Slomnicki
Jixiang Ding
Julia H. Chariker
Eric C. Rouchka
Sujata Saraswat Ohri
Scott R Whittemore
Michal Hetman
Publication Year :
2022
Publisher :
Research Square Platform LLC, 2022.

Abstract

The integrated stress response (ISR)-activated transcription factors ATF4 and CHOP/DDIT3 may regulate oligodendrocyte (OL) survival, tissue damage and functional impairment/recovery in white matter pathologies, including traumatic spinal cord injury (SCI). Accordingly, in OLs of OL-specific RiboTag mice, Atf4, Chop/Ddit3 and their downstream target gene transcripts were acutely upregulated at 2, but not 10, days post-contusive T9 SCI coinciding with maximal loss of spinal cord tissue. Unexpectedly, another, OL-specific upregulation of Atf4/Chop followed at 42 days post-injury. However, wild type vs. OL-specific Atf4-/- or Chop-/- mice showed similar white matter sparing and OL loss at the injury epicenter, as well as unaffected hindlimb function recovery as determined by the Basso mouse scale (BMS). In contrast, the horizontal ladder test revealed persistent worsening or improvement of fine locomotor control in OL-Atf4-/- or OL-Chop-/- mice, respectively. Moreover, chronically, OL-Atf4-/- mice showed decreased walking speed during plantar stepping despite greater compensatory forelimb usage. Therefore, ATF4 supports, while CHOP antagonizes, fine locomotor control during post-SCI recovery. No correlation between those effects and white matter sparing together with chronic activation of the OL ISR suggest that in OLs, ATF4 and CHOP regulate function of spinal cord circuitries that mediate fine locomotor control during post-SCI recovery.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........834200a7f103c7aa49923b17100ab704
Full Text :
https://doi.org/10.21203/rs.3.rs-2184294/v1