Additional file 2 of ADAM22/LGI1 complex as a new actionable target for breast cancer brain metastasis

Additional file 2: Figure S1. Confirmation of LY2 ADAM22 CRISPR knockout cell line. (a) RFP expression confirming CRISPR /Cas9 mediated double stranded break and insertion of an RFP tagged HDR plasmid (b) FACS gating strategy based on high RFP expression to single cell sort CRISPR/Cas9 ADAM22 KO cells (right) from LY2 WT cells (left) (c) Confirmation of ADAM22 gene silencing in an LY2 ADAM22 KO clone (Clone H (C-H)) by PCR and Western blot analysis (n = 4). *** p