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Tartary Buckwheat FtMYB31 Gene Encoding an R2R3-MYB Transcription Factor Enhances Flavonoid Accumulation in Tobacco
- Source :
- Journal of Plant Growth Regulation. 39:564-574
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The R2R3-MYB transcription factors play an important role in regulating secondary metabolism biosynthesis and abiotic stress in plants. In this article, we report the identification of the transcription factor gene, FtMYB31, from the popular Eurasian crop tartary buckwheat (Fagopyrum tataricum) that enhances accumulation of the nutritionally beneficial compound rutin in transgenic tobacco leaves. The FtMYB31 complete cDNA coding sequence was isolated from the leaves of tartary buckwheat, and multiple protein sequence alignments and conserved domain analysis showed it contained a typical R2R3 MYB domain. Subcellular location experiments showed the FtMYB31 protein is localized in nucleus. The phylogenetic tree clustered FtMYB31 with VvMYBPA1 from Vitis vinifera, and AtMYB123 from Arabidopsis thaliana, belonging to the Subgroup 5 cluster. Comparison by qRT-PCR of FtMYB31 transcripts and those from rutin synthesis-related genes showed a relationship between FtMYB31, Ft4CL, and FtUFGT transcripts and rutin content in different tissues of F. tataricum, with correlation coefficients of − 0.68, 0.69, and 0.47, respectively. Transgenic experiments indicated that FtMYB31 upregulated CHS, F3H, and FLS genes in transgenic tobacco and enhanced the accumulation of rutin and total flavonols. These results suggest that FtMYB31 encodes an R2R3-MYB transcription factor that positively regulates flavonol biosynthesis in tartary buckwheat and tobacco and is a possible target for genetically modifying tartary buckwheat to enhance the content of beneficial compounds such as rutin.
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Fagopyrum tataricum
food and beverages
Plant Science
biology.organism_classification
01 natural sciences
03 medical and health sciences
Rutin
chemistry.chemical_compound
030104 developmental biology
Biochemistry
chemistry
Complementary DNA
Arabidopsis thaliana
MYB
Transcription Factor Gene
Agronomy and Crop Science
Transcription factor
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14358107 and 07217595
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Journal of Plant Growth Regulation
- Accession number :
- edsair.doi...........82cdcf934d10e93b29e3aaec1250516b