Spectral identification of melon seeds variety based on k-nearest neighbor and Fisher discriminant analysis

Impurity of melon seeds variety will cause reductions of melon production and economic benefits of farmers, this research aimed to adopt spectral technology combined with chemometrics methods to identify melon seeds variety. Melon seeds whose varieties were "Yi Te Bai", "Yi Te Jin", "Jing Mi NO.7", "Jing Mi NO.11" and " Yi Li Sha Bai "were used as research samples. A simple spectral system was developed to collect reflective spectral data of melon seeds, including a light source unit, a spectral data acquisition unit and a data processing unit, the detection wavelength range of this system was 200-1100nm with spectral resolution of 0.14 ~7.7nm. The original reflective spectral data was pre-treated with de-trend (DT), multiple scattering correction (MSC), first derivative (FD), normalization (NOR) and Savitzky-Golay (SG) convolution smoothing methods. Principal Component Analysis (PCA) method was adopted to reduce the dimensions of reflective spectral data and extract principal components. K-nearest neighbour (KNN) and Fisher discriminant analysis (FDA) methods were used to develop discriminant models of melon seeds variety based on PCA. Spectral data pretreatments improved the discriminant effects of KNN and FDA, FDA generated better discriminant results than KNN, both KNN and FDA methods produced discriminant accuracies reaching to 90.0% for validation set. Research results showed that using spectral technology in combination with KNN and FDA modelling methods to identify melon seeds variety was feasible.