Back to Search
Start Over
Fabrication of high-capacity cation-exchangers for protein adsorption: Comparison of grafting-from and grafting-to approaches
- Source :
- Frontiers of Chemical Science and Engineering. 13:120-132
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- In this work, we have synthesized two polymer-grafted cation exchangers: one via the grafting-from approach, in which sulfopropyl methacrylate (SPM) is grafted through atom transfer radical polymerization onto Sepharose FF (the thus resulting exchanger is referred as Sep-g-SPM), and another via the grafting-to approach, in which the polymer of SPM is directly coupled onto Sepharose FF (the thus resulting exchanger is called as Sep-pSPM). Protein adsorption on these two cation exchangers have been also investigated. At the same ligand density, Sep-g-SPM has a larger accessible pore radius and a smaller depth of polymer layer than SeppSPM, due to the controllable introduction of polymer chains with the regular distribution of the ligand. Therefore, high-capacity adsorption of lysozyme and γ-globulin could be achieved simultaneously in Sep-g-SPM with an ionic capacity (IC) of 308 mmol$L–1. However, Sep-pSPM has an irregular chain distribution and different architecture of polymer layer, which lead to more serious repulsive interaction to proteins, and thus Sep-pSPM has a lower adsorption capacity for γ-globulin than Sep-g-SPM with the similar IC. Moreover, the results from protein uptake experiments indicate that the facilitated transport of adsorbed γ-globulin occurs only in Sep-pSPM and depends on the architecture of polymer layers. Our research provides a clear clue for the development of high-performance protein chromatography.
- Subjects :
- chemistry.chemical_classification
Facilitated diffusion
Chemistry
Atom-transfer radical-polymerization
General Chemical Engineering
fungi
010401 analytical chemistry
Ionic bonding
02 engineering and technology
Polymer
021001 nanoscience & nanotechnology
Methacrylate
01 natural sciences
0104 chemical sciences
Sepharose
Adsorption
Chemical engineering
0210 nano-technology
Protein adsorption
Subjects
Details
- ISSN :
- 20950187 and 20950179
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Frontiers of Chemical Science and Engineering
- Accession number :
- edsair.doi...........81ee5af77c1cf6521d73426077f55deb