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An artificial biofilm induced secondary caries model for in vitro studies
- Source :
- Australian Dental Journal. 56:40-47
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Background: The aim of this study was to establish a new in vitro model for biofilm induced secondary caries studies using an oral biofilm reactor. Methods: An approximately 2 × 3 × 2 mm3 sized dentino-enamel Class I cavity was prepared in the middle of a square-shaped specimen from the mid-labial portion of bovine incisors. The cavities were partially filled with either Clearfil AP-X with SE-Bond or Clearfil AP-X without any bond. Artificial biofilms were then formed on the resin composite filled surfaces using three species of oral bacteria in an oral biofilm reactor for 20 hours followed by 7- or 30-day incubation periods. Results: The lesions were clearly visible on fluorescence microscopy and by scanning electron microscopy in the enamel at the interface of resin restorations in all samples. The data from image analysis showed that the lesion size was largest in the No-bond samples with statistically significant differences (p
- Subjects :
- Enamel paint
business.industry
Chemistry
Scanning electron microscope
medicine.medical_treatment
Biofilm
Dentistry
Penetration (firestop)
Demineralization
visual_art
Microscopy
visual_art.visual_art_medium
Fluorescence microscope
medicine
business
General Dentistry
Dental restoration
Nuclear chemistry
Subjects
Details
- ISSN :
- 00450421
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Australian Dental Journal
- Accession number :
- edsair.doi...........81b9459c63102382a9d5cf9d2b0f24c5