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Effect of sulfated galactan from Porphyra haitanensis on H2O2-induced premature senescence in WI-38 cells
- Source :
- International Journal of Biological Macromolecules. 106:1235-1239
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Porphyran sulfated galactan extracted from red algae Porphyra haitanensis is a sulfated polysaccharide, which possesses excellent activities. In the present study, WI-38 cells were treated with H2O2 to induce premature senescence and then the protection of porphyran against aging in vitro and associated molecular mechanisms were investigated. The protection occurred in a dose-dependent manner, offering an optimal efficacy starting at 10μg/mL. The proportion of SA-β-gal positive cells in porphyran group decreases from 53% to 23% in the cultures at 30 PDs. Porphyran has been detected specifically reducing SAHF-like foci formation in senescent cells. In addition, porphyran significantly affected the p53-p21 pathways in H2O2-treated WI-38 cells. Our data suggest the promising role of porphyran as an attractive and bio-safe agent with the potential to retard senescence and attenuate senescence-related diseases.
- Subjects :
- 0301 basic medicine
Senescence
chemistry.chemical_classification
biology
Porphyran
General Medicine
Red algae
Galactan
biology.organism_classification
Polysaccharide
Biochemistry
WI-38
In vitro
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Structural Biology
Porphyra haitanensis
Molecular Biology
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi...........81240ae5fa88526331438c9e856cbbc7