Extraction of agricultural chemical cartap in human blood

The selection of best solvent for extracting cartap from human blood was examined. The experimental procedures were as follows ; One ml of blood sample was added to 0.5 ml of 8% sodium hydroxide solution in a 15 ml test tube. The sample was extracted with 5 ml of cyclohexane by shaking for 3 min, and centrifuged at 3000 rpm for 2 min. The cartap extracted with cyclohexane was derivatized by reacting with 3, 4-dimethyl benzyl chloride. The 3, 4-dimethyl benzyl derivative of cartap was spectrophotometrically measured by using tropaeoline OO as an ion-associating reagent. For the extraction of cartap from blood sample no interference could be observed with the compounds, such as Fe (III), Cu (II), glycine, L-phenylalanine, urea, glucose, lactose.