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Chemopreventive Properties of Genipin on AGS Cell Line via Induction of JNK/Nrf2/ARE Signaling Pathway
- Source :
- Journal of Biochemical and Molecular Toxicology. 30:45-54
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Roles of dietary phytochemicals in cancer chemoprevention via induction of nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated antioxidant enzymes have been well established in a number of studies. In this study, FACS analysis was used to reveal that the intracellular reactive oxygen species level decreased at 0-25 μM of genipin treatment. Furthermore, immunofluorescence analysis and Western blotting were used to demonstrate that genipin treatment resulted in the upregulation and nuclear translocation of Nrf2, as well as upregulation of gastrointestinal glutathione peroxidase. Finally, we found that C-Jun-NH2-kinase (JNK) was also dose-dependently activated, where depleting JNK by using a biochemical inhibitor indicated that JNK was upstream of Nrf2. Interestingly, the antioxidant effects were limited to the treatment in the lower dosage of genipin, where higher dosage of genipin treatment resulted in the increased reactive oxygen species level and cytotoxicity. Thus, this study demonstrates for the first time that lower dosage of genipin results in the induction of JNK/Nrf2/ARE signaling pathway and protection from cell death.
- Subjects :
- 0301 basic medicine
Programmed cell death
Antioxidant
Health, Toxicology and Mutagenesis
medicine.medical_treatment
Pharmacology
Biology
Toxicology
medicine.disease_cause
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Downregulation and upregulation
medicine
Cytotoxicity
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
General Medicine
030104 developmental biology
chemistry
Genipin
Molecular Medicine
Signal transduction
Oxidative stress
Subjects
Details
- ISSN :
- 10956670
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Journal of Biochemical and Molecular Toxicology
- Accession number :
- edsair.doi...........80ac35685eb980f6abaa35730abdff8f