Isolation and Identification of Enzymes that are Responsible for the Isolation of Plant Protoplasts

Plant protoplasts are isolated enzymically by the use of commercial cell wall degrading enzymes. These preparations are crude enzymes and, therefore, contain some toxic or undesirable substances. It is expected that highly purified enzymes may be useful for the isolation of intact protoplasts. However, it is not known what kinds of enzymes are actually required for the isolation of plant protoplasts. The answer to this question would be a prerequisite for the utilization of highly purified enzymes for the isolation.