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Physiological Analysis Reveals the Possible Resistance Mechanisms of Glycine max to Fusarium solani
- Source :
- Journal of Agricultural Science. 10:44
- Publication Year :
- 2018
- Publisher :
- Canadian Center of Science and Education, 2018.
-
Abstract
- Sudden death syndrome (SDS) of soybean is a complex root rot disease caused by the semi-biotrophic fungus Fusarium solani (F. solani) and a leaf scorch disease; caused by toxins produced by pathogen in the roots. However, the mechanism of soybean resistant to F. solani is still poorly understood. Eighteen soybean cultivars were screened for SDS resistance, with one cultivar showing susceptibility and one cultivar showing resistance to F. solani infection. Histochemical analysis with diaminobenzidine (DAB) and Trypan blue staining indicated an accumulation of reactive oxygen species (ROS) and cell death in surrounding area of SDS which was higher in susceptible cultivar than in resistant cultivar. Furthermore, exogenous salicylic acid (SA) application also induced some level of resistance to F. solani by the susceptible cultivar. A biochemical study revealed that the activities of superoxide dismutase (SOD), peroxidase (POD), and enzymes involved in scavenging ROS, increased in susceptible cultivar after SDS infection. In addition, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content also increased in the susceptible cultivar than in resistant cultivar. High-performance liquid chromatography (HPLC) analysis indicated that free and total salicylic acid (SA) content increased in the susceptible cultivar than in resistant cultivar. In addition, a real-time quantitative PCR analysis showed an accumulation of pathogen related (PR) genes in the resistant cultivar than in susceptible cultivar. Our results show that (i). F. solani infection can increase endogenous SA levels, antioxidase activities, ROS and cell death in susceptible soybean cultivar to induce resistance against Fusarium solani. (ii). F. solani infection induced the expression of SA marker genes in resistant soybean cultivar to enhance resistance.
- Subjects :
- 0106 biological sciences
0301 basic medicine
chemistry.chemical_classification
Reactive oxygen species
biology
fungi
food and beverages
Leaf scorch
biology.organism_classification
medicine.disease
01 natural sciences
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Horticulture
030104 developmental biology
chemistry
biology.protein
Root rot
medicine
Cultivar
Fusarium solani
Salicylic acid
010606 plant biology & botany
Peroxidase
Subjects
Details
- ISSN :
- 19169760 and 19169752
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural Science
- Accession number :
- edsair.doi...........7ef8d3446df660d37e96a949f563ce7e