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Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model
- Source :
- Methods in Molecular Biology ISBN: 9781071607039
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477-89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.
- Subjects :
- Genetically modified mouse
0303 health sciences
Xenotransplantation
medicine.medical_treatment
Strain (biology)
Biology
Molecular biology
Liver regeneration
03 medical and health sciences
Human hepatocyte
0302 clinical medicine
030220 oncology & carcinogenesis
medicine
NSG mouse
Allele
Gene
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-07-160703-9
- ISBNs :
- 9781071607039
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071607039
- Accession number :
- edsair.doi...........7eeebe61b5b84554cfe378e1ed04bc36