Back to Search
Start Over
Retinoic acid effects on an SV-40 large T antigen immortalized adult rat bone cell line
- Source :
- Journal of Cellular Physiology. 179:267-275
- Publication Year :
- 1999
- Publisher :
- Wiley, 1999.
-
Abstract
- Clonal cell lines were established from adult rat tibia cells immortalized with SV-40 large T antigen. One clone (TRAB-11), in which retinoic acid (RA) induced alkaline phosphatase (AP) activity, was selected for further study. The TRAB-11 cells express high levels of type I collagen mRNA, type IV collagen, fibronectin, practically no type III collagen, little osteopontin, and no osteocalcin. RA stimulates proliferation of TRAB-11 cells (starting at 10 pM) and survival (starting at 100 pM). TRAB-11 cells synthesize fibroblast growth factor-2 (FGF-2), which has potent autocrine mitogenic effects on these cells and acts synergistically with RA. TRAB-11 cells attach better to type IV collagen than to fibronectin or laminin. Cell attachment to type IV collagen is increased by RA and decreased (65%) by an antibody directed against α1β1 integrin. RA up-regulates steady-state levels of α1 mRNA without affecting β1 mRNA expression. In conclusion, we report the establishment of a clonal cell line from the outgrowth of adult rat tibiae which is highly sensitive to RA in its growth and survival in culture, apparently as a result of integrin-mediated cell interaction with extracellular matrix proteins. J. Cell. Physiol. 179:267–275, 1999. © 1999 Wiley-Liss, Inc.
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 179
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi...........7ed782cc291821944eb060ad89206728