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Studies on locust rectum. III. Stimulation of electrogenic chloride transport by hemolymph
- Source :
- Canadian Journal of Zoology. 58:1933-1939
- Publication Year :
- 1980
- Publisher :
- Canadian Science Publishing, 1980.
-
Abstract
- Hemolymph was collected from recently fed desert locusts either by adsorption onto filter paper, or by centrifugation and methanol extraction. Whole hemolymph caused both the short-circuit current (Isc) and open-circuit transepithelial electropotential difference (PD) across locust recta mounted in Ussing-type chambers to double during the steady-state period. Methanol extracts of hemolymph caused similar but smaller increases in Isc. The transepithelial resistance (R) did not change. Simultaneous measurements of 36Cl− fluxes indicated that all of the increase in Isc following stimulation could be accounted for by a parallel increase in net Cl− absorption from the lumen side. With the exception of an initial small biphasic fluctuation in Isc, stimulation by hemolymph exhibited identical characteristics to those produced by submaximal dosages of corpora cardiaca (CC). Cardiatectomy drastically reduced the stimulatory activity of hemolymph, suggesting that this neuroendocrine organ is the source of the active factor (chloride transport stimulating hormone (CTSH)) in hemolymph.
- Subjects :
- medicine.medical_specialty
Chromatography
Filter paper
biology
Extraction (chemistry)
Stimulation
biology.organism_classification
Chloride
Endocrinology
Internal medicine
Hemolymph
medicine
Animal Science and Zoology
Centrifugation
Ecology, Evolution, Behavior and Systematics
Locust
medicine.drug
Subjects
Details
- ISSN :
- 14803283 and 00084301
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Zoology
- Accession number :
- edsair.doi...........7df41e2cad6ae73f897965d31cfaaf42