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Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser-Induced Fluorescence Detection

Authors :
Romana Konecna
Iva Blazkova
Ondrej Zitka
Vojtech Adam
Maja Stanisavljevic
Tomas Eckschlager
Hoai Viet Nguyen
Marketa Vaculovicova
Sona Krizkova
René Kizek
Marie Stiborová
Source :
Chromatographia. 77:1469-1476
Publication Year :
2014
Publisher :
Springer Science and Business Media LLC, 2014.

Abstract

Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4–10. Two times higher fluorescence intensity was observed at pH 4 compared to pH 10. Further, the DOX behavior in capillary electrophoresis (CE) was investigated. Electrophoretic mobilities (CE) in various pH of the background electrolyte were determined within the range from 16.3 to −13.3 × 10 −9 m−2 V−1 s−1. Finally, CE was also used to monitor the encapsulation of DOX into the cavity of apoferritin as well as the pH-triggered release.

Details

ISSN :
16121112 and 00095893
Volume :
77
Database :
OpenAIRE
Journal :
Chromatographia
Accession number :
edsair.doi...........7de8354ad406ccddcd5ed7f4bdc1c293
Full Text :
https://doi.org/10.1007/s10337-014-2733-6