Light absorption and fluorescence of highly diluted chlorophyll solutions

The absorption and fluorescence spectra 10−4, 6·10−6 and 10−7 M chlorophyll-a and chlorophyll-b solutions were investigated in 9 solvents. The position of the bands, the molar extinction coefficients, the ratios of the heights of the blue and the red peak, the Stokes’ shift of fluorescence and absorption bands were measured, the oscillator strength of the red band was calculated and the vibrational temperature calculated from Stepanov’s relation between the absorption and fluorescence spectra.