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Enrichment and Identification of Metallothionein by Functionalized Nano-Magnetic Particles and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
- Source :
- Chinese Journal of Analytical Chemistry. 46:1069-1076
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- As a low molecular weight protein with the ability of binding metal ions and high inducibility, metallothionein (MT) is often regarded as an important biomarker for assessment of heavy metal pollution in water environment. In the light of that the traditional process of enrichment and identification is time-consuming and complicated, we prepared a core-shell nanoparticle, gold-coated iron oxide nanoparticles (Fe3O4@Au NPs) herein. It possessed the advantages of fast response to magnetic fields and optical properties attributing to Fe3O4 and Au nanoparticles, respectively. The Fe3O4@Au nanoparticles could be used to enrich MT simply through Au–S interaction, and the purified proteins were determined by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The results showed that the Fe3O4@Au nanoparticles could directly enrich MT from complex solutions and the detection limit could be as low as 10 fg mL−1.
- Subjects :
- Detection limit
Chemistry
Metal ions in aqueous solution
010401 analytical chemistry
Inorganic chemistry
Nanoparticle
02 engineering and technology
021001 nanoscience & nanotechnology
Mass spectrometry
01 natural sciences
0104 chemical sciences
Analytical Chemistry
Matrix-assisted laser desorption/ionization
chemistry.chemical_compound
Water environment
Magnetic nanoparticles
0210 nano-technology
Iron oxide nanoparticles
Subjects
Details
- ISSN :
- 18722040
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Analytical Chemistry
- Accession number :
- edsair.doi...........7bf533307425be8a468b7085593ca196