Chronic toxicity of three neonicotinoid insecticides and their mixture on two daphniid species: Daphnia magna and Ceriodaphnia dubia

Neonicotinoid insecticides represent nearly a quarter of the global market and are widely used in agriculture but also for lawn, garden care, and pest control. They are highly water-soluble, persistent in soil, and may enter the aquatic compartment via spray drift, runoff, or leaching, and contribute to downstream aquatic toxicity. Although insects appear to be the most sensitive group to neonicotinoids, other groups, such as crustaceans and birds, may also be affected. Furthermore, most studies focus on single-insecticide exposure and very little is known concerning the impact of neonicotinoid mixtures on aquatic invertebrates. The present study was designed to test potential toxicological effects of an environmentally relevant mixture of imidacloprid, clothianidin, and thiamethoxam on populations of Ceriodaphnia dubia and Daphnia magna under controlled conditions. Chronic toxicity tests were conducted in the laboratory, and survival and reproduction were measured for both species under exposure to nominal concentrations of imidacloprid (0.256 µg/L), clothianidin (3.11 µg/L), thiamethoxam (1.49 µg/L), and a mixture of the three compounds at the same concentrations of the individual compounds. The neonicotinoids did not affect the survival of C. dubia and D. magna founders. Reproduction of C. dubia was affected only by the mixture. All three individual insecticides as well as the mixture caused a significant reduction in the reproduction of D. magna. Our results highlight the complexity of pesticide toxicity and show that traditional toxicological approaches such as acute mortality studies, especially tests with single compounds, can underestimate negative impacts that occur in the environment.HighlightsNeonicotinoids are currently the most frequently used insecticides worldwide.An environmentally relevant mixture of three neonicotinoids was evaluated on two daphniid species.The mixture negatively affected the reproduction of C. dubia and Daphnia magna.Traditional toxicological approaches with single compounds may underestimate the effects occurring in the environment at low concentrations.