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A Simple Paper-Based α-Amylase Separating System for Potential Application in Biological Sciences
- Source :
- BioChip Journal. 15:252-259
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- The salivary glands are closely associated with stress conditions and the neuroendocrine system. The concentrations of these salivary biomarkers fluctuate with a person’s disease status. Until now, salivary diagnostics have been promising; however, the analytical tools for detecting salivary biomarkers have remained burdensome due to the lack of user-friendly and cost-effective equipment. In this study, we used a microfluidic starch-coated paper-based chip to isolate ɑ-amylase from human saliva. The concentration of ɑ-amylase on the head and tail sections of the paper-based chip was compared in the presence or absence of starch. We found that ɑ-amylase was highly concentrated only in the head section of the starch-coated paper-based chip through Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). We expect our designed paper-based chip will aid in α-amylase detection technologies using saliva and small amounts of other biofluids including blood, tears, pancreatic juice, and urine.
- Subjects :
- Saliva
Disease status
biology
Chemistry
010401 analytical chemistry
Biomedical Engineering
Bioengineering
02 engineering and technology
Paper based
021001 nanoscience & nanotechnology
01 natural sciences
0104 chemical sciences
Biochemistry
Salivary diagnostics
Pancreatic juice
biology.protein
Amylase
Stress conditions
Electrical and Electronic Engineering
0210 nano-technology
Biological sciences
Biotechnology
Subjects
Details
- ISSN :
- 20927843 and 19760280
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- BioChip Journal
- Accession number :
- edsair.doi...........7b9459173df76bccd291b12e2148e5dc