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Development and design of up-to-date laser scanning two-photon microscope using in neuroscience
- Source :
- SPIE Proceedings.
- Publication Year :
- 2017
- Publisher :
- SPIE, 2017.
-
Abstract
- Today one of the main areas of application of two-photon microscopy is biology. This is due to the fact that this technique allows to obtain 3D images of tissues due to laser focus change, that is possible due to substantially greater penetration depth on the main wavelength into biological tissues. Self-developed microscopy system provides possibility to service it and modify the structure of microscope depending on highly specialized experimental design and scientific goals. This article may be regarded as a quick reference to laboratory staff who are wishing to develop their own microscopy system for self-service and modernization of the system and in order to save the lab budget.
- Subjects :
- Focus (computing)
Microscope
Materials science
Laser scanning
business.industry
02 engineering and technology
Laser
law.invention
Optics
Two-photon excitation microscopy
law
Microscopy
0202 electrical engineering, electronic engineering, information engineering
Confocal laser scanning microscopy
020201 artificial intelligence & image processing
business
Penetration depth
Subjects
Details
- ISSN :
- 0277786X
- Database :
- OpenAIRE
- Journal :
- SPIE Proceedings
- Accession number :
- edsair.doi...........7b18dde0aac9970cf5f6e549a8b74a25