Genetic polymorphism of the growth hormone gene and their effects on growth metrics in the Assam Hill and Sirohi goats under subtropical climatic condition

Growth hormone (GH) is a gene that regulates milk production, reproduction, and growth in animals. Therefore, the purpose of this study was to identify GH gene variations and their associations with growth metrics in Sirohi and Assam hill goats. We identified two polymorphic loci (GH1-HaeIII & GH2-HaeIII) which were connected to growth attributes in 161 animals of Assam hill (n = 114) and Sirohi (n = 47) goats using the methods of PCR-RFLP. According to PCR-RFLP results, the GH gene's GH1-HaeIII locus contains two alleles (A and B) and two genotypes (AA and AB), whereas the GH2-HaeIII locus contains two alleles (C and D) and two genotypes (CC and CD). In the two goat breeds, the allele frequencies of A and B were 0.36 − 0.28 and 0.64–0.72, respectively, while the frequencies of alleles C and D were 0.71 − 0.70 and 0.29–0.30. The polymorphic loci were demonstrated to be in a state of Hardy-Weinberg disequilibrium in both breeds of goats (P