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Soluble cytokine-receptors are present in normal human urine
- Source :
- Cytokine. 1:149
- Publication Year :
- 1989
- Publisher :
- Elsevier BV, 1989.
-
Abstract
- Affinity chromatography of crude human urinary proteins on either human rIL-6, human rIFN-gamma, or anti-IFN-gamma-R mAb yielded the two respective soluble receptors in significant quantities. A single sequence of 30 amino acid residues was obtained by NH2-terminal microsequencing of the protein peak purified in tandem by affinity chromatography on an IL-6 column and reversed-phase HPLC. This sequence was identical to the predicted NH2-terminal sequence of IL-6-R as previously reported. Analysis of the eluted proteins from both IFN-gamma and anti-IFN-gamma-R columns by inhibition of solid phase RIA, ELISA, SDS-PAGE, and Western blotting proved the existence of soluble IFN-gamma-R in normal urine. Our finding, together with the already known presence of urinary TNF binding proteins and a soluble IL-2-R both in plasma and in urine, indicates that release of soluble cytokine receptors into body fluids is a general phenomenon that occurs under normal physiological conditions.
Details
- ISSN :
- 10434666
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- Cytokine
- Accession number :
- edsair.doi...........7a9e83e69e48703c0e764b995c39fc11