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Wing Membrane Biopsies for Bat Cytogenetics: Finding of 2n = 54 in Irish Rhinolophushipposideros (Rhinolophidae, Chiroptera, Mammalia) Supports Two Geographically Separated Chromosomal Variants in Europe
- Source :
- Cytogenetic and Genome Research. 148:279-283
- Publication Year :
- 2016
- Publisher :
- S. Karger AG, 2016.
-
Abstract
- In Europe, 2 different diploid chromosome numbers, 2n = 54 and 2n = 56, have been described in the lesser horseshoe bat (Rhinolophushipposideros). The eastern form with 2n = 56 extends from the Czech Republic to Greece. To date, specimens with 54 chromosomes have been reported only from Spain and Germany. This study expands the distributional area of the western variant to Ireland. Strikingly, this distribution of European chromosomal variants is in contrast to the available molecular data that indicate little genetic differentiation of R. hipposideros populations spanning Northwestern to Central Europe. Further, we have developed an optimized protocol for establishing fibroblast cell cultures, suitable for karyotype analyses, from 3-mm wing membrane biopsies. This is a useful technique for cytogenetic studies of endangered bat species, as this non-lethal sampling method imposes only minimum stress to the animal without lasting adverse effects and is routinely used to sample tissue probes for molecular genetic studies in bats.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
biology
Cytogenetics
Endangered species
Chromosome
Zoology
Karyotype
Horseshoe bat
biology.organism_classification
Hipposideros
03 medical and health sciences
030104 developmental biology
Genetics
medicine
Ploidy
Molecular Biology
Genetics (clinical)
Wing membrane
Subjects
Details
- ISSN :
- 1424859X and 14248581
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Cytogenetic and Genome Research
- Accession number :
- edsair.doi...........7a78c82fa68916bb0e1f9d1081b5da58