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Specific-Locus Amplified Fragment Sequencing (SLAF-Seq) as High-Throughput SNP Genotyping Methods
- Source :
- Methods in Molecular Biology ISBN: 9781071612002
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- Most plant agronomic traits are quantitatively inherited. Identification of quantitative trait loci (QTL) is a challenging target for most scientists and crop breeders as large-scale genotyping is difficult. Molecular marker technology has continuously evolved from hybridization-based technology to PCR-based technology, and finally, sequencing-based high-throughput single-nucleotide polymorphisms (SNPs). High-throughput sequencing technologies can provide strategies for sequence-based SNP genotyping. Here we describe the SLAF-seq that can be applied as the SNP genotyping approach. The high-throughput SNP genotyping methods will prove useful for the construction of high-density genetic maps and identification of QTLs for their deployment in plant breeding and facilitate genome-wide selection (GWS) and genome-wide association studies (GWAS).
- Subjects :
- 0106 biological sciences
0301 basic medicine
fungi
food and beverages
Locus (genetics)
Genome-wide association study
Computational biology
Quantitative trait locus
Biology
01 natural sciences
SNP genotyping
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Molecular marker
SNP
Genotyping
010606 plant biology & botany
Genetic association
Subjects
Details
- ISBN :
- 978-1-07-161200-2
- ISBNs :
- 9781071612002
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071612002
- Accession number :
- edsair.doi...........7a0a73c2b345d00cccb3bc93b8ce80ba