Transglycosylation reaction and raw starch hydrolysis by novel carbohydrolase fromLipomyces starkeyi

A novel carbohydrolase, which is a DXAMase, containing both dextranase and amylase equivalent activities, was purified fromLipomyces starkeyi KSM22. The purified DXAMase was also found to hydrolyze cellobiose, gentiobiose, trehalose and melezitose, while disproportionation reactions were exhibited with various di-and tri-saccharides, such as maltose, isomaltose, gentiobiose, kojibiose, sophorose, panose, maltotriose, and isomaltotriose with various kinds of oligosaccharides produced as acceptor reaction products. Furthermore, the purified DXAMase hydrolyzed raw waxy rice starch and produced maltodextrin to the extent of 50% as a glucose equivalent.