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The effect of cell size on the kinetics of yeast disintegration in a bead mill
- Source :
- Powder Technology. 380:584-597
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The results of modelling the effect of cell sizes on the microorganism disintegration process are presented. Experiments were conducted in a horizontal bead mill for baker's yeast Saccharomyces cerevisiae. The kinetics of the cell disruption process was investigated by optical image analysis and the release of intracellular protein by Bradford method. A large variation in the size of yeast from one production cycle has been demonstrated. A significant increase in the constant rate of the process with an increase in the range of the cell size class was demonstrated. More beneficial values of the coefficient of determination were obtained for the spherical model of the cell. Worse results of the process description were obtained for the ellipsoidal model. They may result from large variations in cell volume in individual size classes. The greatest changes in the cell Elongation parameter within one size class ranged from 1.00 to 1.54.
- Subjects :
- Coefficient of determination
biology
Chemistry
General Chemical Engineering
Microorganism
Saccharomyces cerevisiae
Kinetics
02 engineering and technology
021001 nanoscience & nanotechnology
biology.organism_classification
Yeast
020401 chemical engineering
Volume (thermodynamics)
Biophysics
Cell disruption
0204 chemical engineering
0210 nano-technology
Bradford protein assay
Subjects
Details
- ISSN :
- 00325910
- Volume :
- 380
- Database :
- OpenAIRE
- Journal :
- Powder Technology
- Accession number :
- edsair.doi...........79abd8f5299ce69805ca02d48678e986