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Ionic Strength-Dependent Proteolytic Activation of Protein Kinase C by Trypsin-Like Protease1
- Source :
- The Journal of Biochemistry. 104:934-938
- Publication Year :
- 1988
- Publisher :
- Oxford University Press (OUP), 1988.
-
Abstract
- Protein kinase C, reversibly bound to rat liver plasma membrane through Ca2+, was activated by endogenous trypsin-like protease in an ionic strength-dependent manner. In an attempt to understand the reaction mechanism, the EGTA-extracted protein kinase C and the trypsin-like protease (Tanaka, K. et al. (1986) J. Biol. Chem. 261, 2610-2615) were separately purified from plasma membrane. In the reaction system using these purified enzymes, increasing the ionic strength with NaCl (140-210 mM) effectively enhanced the proteolytic activation of the protein kinase C in the presence of Ca2+ and phospholipid. These results suggest that ionic strength is an important factor for the proteolytic activation of membrane-bound rat liver protein kinase C.
Details
- ISSN :
- 17562651 and 0021924X
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- The Journal of Biochemistry
- Accession number :
- edsair.doi...........792c19b761a1f8b3a3558c8946a72174