Identification of inconsistentS-genotypes in 15 Chinese pear (PyrusL.) cultivars

SummaryMost pear (Pyrus L.) cultivars exhibit gametophytic self-incompatibility (GSI), controlled by a single S-locus. Crosscompatible cultivars with different S-genotypes must be inter-planted in an orchard for efficient fruit production.Thus, it is essential to identify S-genotypes in order to choose pollinators for fruit production. The S-genotypes of many Chinese pear cultivars have been identified, but some results remain inconsistent. In this study, 15 Chinese pear cultivars with inconsistent S-genotypes were selected for further identification using 37 leaf samples collected from six different regions in China. The S-genotypes of all 15 pear cultivars were determined by polymerase chain reaction (PCR), cloning, and sequencing of the PCR products. The S-genotypes were as follows: ‘Baozhuli’ (S22S42), ‘Cangxixueli’ (S5S15), ‘Hanxiang’ (S12S31), ‘Hangqing’ (S1S4), ‘Jinqiuli’ (S3S9), ‘Jinfeng’ (S17S19), ‘Longxiang’ (S16S42), ‘Nanguoli’ (S11S17), ‘Pingxiangli’ (S31Sd), ‘Qingkui’ (S1S3), ‘Qingxiang’ (S4S...