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Rapid and facile detection of HBV with CRISPR/Cas13a

Authors :
Ronghua Ding
Yue Shen
Mingzhu Yuan
Xue Zheng
Shuaiyin Chen
Guangcai Duan
Source :
New Journal of Chemistry. 46:19997-20004
Publication Year :
2022
Publisher :
Royal Society of Chemistry (RSC), 2022.

Abstract

A rapid point-of-care assay based on CRISPR-Cas13a for HBV was built with a LOD of 1 copy per μL was achieved in 50 min. The lateral flow test strip method can achieve naked-eye results, and the fluorescence readout can achieve real-time detection.

Details

ISSN :
13699261 and 11440546
Volume :
46
Database :
OpenAIRE
Journal :
New Journal of Chemistry
Accession number :
edsair.doi...........784f462d7da0ae7cd7e4bb3795629da1