Abstract A038: Assessment of angiogenesis markers in advanced cervical carcinoma patients that received chemoradiation therapy plus/minus neoadjuvant chemotherapy

The expression profile of angiogenesis associated proteins was assessed in patients with locally advanced cervical cancer included in a prospective, randomized phase II study. The majority of patients had squamous cell carcinoma (87.8%). Group A individuals were treated with neoadjuvant chemotherapy (cisplatin and gemcitabine) (NAC), followed by chemoradiation with cisplatin (CRT). Group B patients received CRT. Serum samples from patients in group A were collected at 03 different time points (at baseline (n=34), after NAC (n=24), and after CRT (n=27)). Samples of group B were collected in 02 different time points (at baseline (n=40) and after CRT (n=34)). Samples were grouped and evaluated with a non-parametric Mann-Whitney test where we considered the points as independent groups. The Friedman test (ANOVA) was used for analysis of group A (with three evaluation points). The levels of different proteins associated with angiogenesis (EGF, angiopoietin, G-CSF, endothelin-1, FGF-1, follistatin, IL-8, HGF, HB-EGF, PLGF, VEGF- D, VEGF-A and FGF-2) were assessed by a Multiplex Assay using the Luminex® technology. HB-EGF, IL-8 and VEGF-C were significantly decreased in patients from group A, whereas all others biomarkers did not present intra or intergroup differences. NAC was associated with an inferior PFS (progression free survival), with 3-year PFS rates of 40.9% compared with 60.4% in the CRT arm (HR, 1.84; 95% CI, 1.04 – 3.26; P = .033). NAC was also associated with a lower OS (overall survival) (3-year OS, 60.7% vs 86.8%; HR, 2.79; 95% CI, 1.29-6.01; P=0.006). After treatment completion, complete response rates were 56.3% in the NAC arm and 80.3% in the CRT arm (P=0.008). Toxicities were similar in both arms, with the exception of hypomagnesemia and neuropathy that were more commonly observed within NAC individual. We can conclude, within the limitations of presented study, that the NAC in group A results in significant alterations in specific markers associated with angiogenesis. Citation Format: ROBERTO JUN ARAI, THIAGO RODRIGUES DA COSTA, SILVANEIDE FERREIRA, LAURA SICHERO, MARIA PILAR ESTEVEZ DIZ. Assessment of angiogenesis markers in advanced cervical carcinoma patients that received chemoradiation therapy plus/minus neoadjuvant chemotherapy [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; 2019 Oct 26-30; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2019;18(12 Suppl):Abstract nr A038. doi:10.1158/1535-7163.TARG-19-A038