Cytotoxicity evaluation and physicochemical properties of collagen isolated from silver carp tails as potential biomaterial

Collagen is widely used as a biomaterial in the pharmaceutical and cosmetic industries, in the production of hydrogels, wound dressings, bioactive nano/microfibers, controlled drug delivery systems, etc. The collagen isolated from the aquatic source has a higher biological activity and low risk of transmitting genetic diseases. In recent years the sustainable socio-economic and environmental principles promote the full use of natural resources. Thus, fish collagen extracted from fish by-products (skin, scales, bones and fins) can be valorized as a new collagen alternative source. In this work the enzymatic hydrolysis with pepsin of collagen isolation from silver carp (Hypophthalmichthys molitrix) tails fish has been investigated. We successfully isolated type I collagen with 90-95% purity as determined by FTIR, UV-Vis, EDX and SDS-PAGE analyses. The cytotoxicity of obtained collagen was evaluating by MTS assays.