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Cross-Talk Calibrating Based on Correlation Analysis in High-Throughput Genome Sequencing
- Source :
- 2010 4th International Conference on Bioinformatics and Biomedical Engineering.
- Publication Year :
- 2010
- Publisher :
- IEEE, 2010.
-
Abstract
- The images, in high-throughput genome sequencing of sequencing by synthesize, consist of A, C, G, T base fluorescence images, and the fluorophore emission spectra partially overlap, which causes cross-talk problem. This paper presents a method of correlation analysis, construct the calibration matrix to eliminate interference of the fluorophore. The experiment result shows the method provided in this article is effective to correct cross-talk influence, and get the deconvolution of the clusters signal.
Details
- Database :
- OpenAIRE
- Journal :
- 2010 4th International Conference on Bioinformatics and Biomedical Engineering
- Accession number :
- edsair.doi...........76b4ce2d6a3fa006bebaae4f0891d796