HTAPP_Dissociation of human primary lung cancer resection to a single-cell suspension for single-cell RNA-seq v2

This protocol is used for the dissociation of human primary lung cancer resections to a single-cell suspension compatible with droplet-based single-cell RNA-Seq technology. For the Human Tumor Atlas Pilot Project (HTAPP) it has been successfully applied to non-small cell adenocarcinoma from which it was able to capture a diversity of cell types, including malignant (epithelial) and non malignant cells such asmesenchymal, endothelial, myeloid, and lymphoid cells. In some instances, this protocol can be combined with a protocol for CD45-cell depletion, which is used for fast depletion of immune cells for enrichment of malignant and stromal cells. Description of this protocol and guidance for testing and selecting methods for processing other tumor and sample types can be found in Slyper et al.